easyseptm human epcam positive selection kit Search Results


easyseptm human epcam positive selection kit  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc easyseptm human epcam positive selection kit
( A ) The experimental design and the CNA genetic landscape of tumors in patients St23784/17 and Ti41749/17, showing that St23784/17 had amplifications at the 3q, 6q, 8q, 9q, and 10q22.1 regions of stemness gene localization (the following genes were amplified: SOX2 , MYC , KLF4 , NOTCH1 , NODAL ), and Ti41749/17 had no stemness gene amplifications. ( B – E ) Analysis of the quantitative and qualitative expression of cells obtained from tumor tissue of breast cancer patients. The cells were analyzed by flow cytometry using a FACSCanto II (Becton Dickinson, USA) and FACS Diva software, using CD326 (PerCP-Cy5.5), CD24 (PE-Cy 7), and CD44 (APC) (BD Biosciences, USA) antibodies. (B) Histogram of the IgG1 PerCP Cy5.5 isotope control; (C) isotype control dot-plot for IgG2a PE-Cy 7/IgG2b APC; (D) phenotypic confirmation and qualitative analysis of CD326 (PerCP-Cy5.5) expression in the histogram; (E) phenotypic confirmation and qualitative analysis of CD326 + CD44 + CD24 -\low expression in the dot-plot. ( F ) The dynamics of the <t>EpCam+</t> tumor cell mass increase (percentage of the initial number of cells in culture). ( G ) Nonadherent mammospheres of EpCam + cancer cell cultures (St23784/17 patient). Mammosphere formation within 10 days. Scale bar, 200 μm. ( H ) EpCam + cells without mammosphere formation (patient Ti41749/17). ( I ) Thermal map of stemness gene expression in the environment enriched with EpCam+ tumor cells from the patients St23784/17 and Ti41749/17. ( J ) The content of tumor stem cells in the EpCam + cell enriched environment after a cycle of cultivation and IL-6 treatment (% of the initial cell content in each population).
Easyseptm Human Epcam Positive Selection Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) The experimental design and the CNA genetic landscape of tumors in patients St23784/17 and Ti41749/17, showing that St23784/17 had amplifications at the 3q, 6q, 8q, 9q, and 10q22.1 regions of stemness gene localization (the following genes were amplified: SOX2 , MYC , KLF4 , NOTCH1 , NODAL ), and Ti41749/17 had no stemness gene amplifications. ( B – E ) Analysis of the quantitative and qualitative expression of cells obtained from tumor tissue of breast cancer patients. The cells were analyzed by flow cytometry using a FACSCanto II (Becton Dickinson, USA) and FACS Diva software, using CD326 (PerCP-Cy5.5), CD24 (PE-Cy 7), and CD44 (APC) (BD Biosciences, USA) antibodies. (B) Histogram of the IgG1 PerCP Cy5.5 isotope control; (C) isotype control dot-plot for IgG2a PE-Cy 7/IgG2b APC; (D) phenotypic confirmation and qualitative analysis of CD326 (PerCP-Cy5.5) expression in the histogram; (E) phenotypic confirmation and qualitative analysis of CD326 + CD44 + CD24 -\low expression in the dot-plot. ( F ) The dynamics of the EpCam+ tumor cell mass increase (percentage of the initial number of cells in culture). ( G ) Nonadherent mammospheres of EpCam + cancer cell cultures (St23784/17 patient). Mammosphere formation within 10 days. Scale bar, 200 μm. ( H ) EpCam + cells without mammosphere formation (patient Ti41749/17). ( I ) Thermal map of stemness gene expression in the environment enriched with EpCam+ tumor cells from the patients St23784/17 and Ti41749/17. ( J ) The content of tumor stem cells in the EpCam + cell enriched environment after a cycle of cultivation and IL-6 treatment (% of the initial cell content in each population).

Journal: Oncotarget

Article Title: Amplifications of stemness genes and the capacity of breast tumors for metastasis

doi: 10.18632/oncotarget.27608

Figure Lengend Snippet: ( A ) The experimental design and the CNA genetic landscape of tumors in patients St23784/17 and Ti41749/17, showing that St23784/17 had amplifications at the 3q, 6q, 8q, 9q, and 10q22.1 regions of stemness gene localization (the following genes were amplified: SOX2 , MYC , KLF4 , NOTCH1 , NODAL ), and Ti41749/17 had no stemness gene amplifications. ( B – E ) Analysis of the quantitative and qualitative expression of cells obtained from tumor tissue of breast cancer patients. The cells were analyzed by flow cytometry using a FACSCanto II (Becton Dickinson, USA) and FACS Diva software, using CD326 (PerCP-Cy5.5), CD24 (PE-Cy 7), and CD44 (APC) (BD Biosciences, USA) antibodies. (B) Histogram of the IgG1 PerCP Cy5.5 isotope control; (C) isotype control dot-plot for IgG2a PE-Cy 7/IgG2b APC; (D) phenotypic confirmation and qualitative analysis of CD326 (PerCP-Cy5.5) expression in the histogram; (E) phenotypic confirmation and qualitative analysis of CD326 + CD44 + CD24 -\low expression in the dot-plot. ( F ) The dynamics of the EpCam+ tumor cell mass increase (percentage of the initial number of cells in culture). ( G ) Nonadherent mammospheres of EpCam + cancer cell cultures (St23784/17 patient). Mammosphere formation within 10 days. Scale bar, 200 μm. ( H ) EpCam + cells without mammosphere formation (patient Ti41749/17). ( I ) Thermal map of stemness gene expression in the environment enriched with EpCam+ tumor cells from the patients St23784/17 and Ti41749/17. ( J ) The content of tumor stem cells in the EpCam + cell enriched environment after a cycle of cultivation and IL-6 treatment (% of the initial cell content in each population).

Article Snippet: The EpCam + cell fraction was isolated using the EasySepTM Human EpCam Positive Selection Kit (StemCell Technologies, Catalog #18356).

Techniques: Amplification, Expressing, Flow Cytometry, Software, Control, Gene Expression

Stem cell populations (CD326+ fraction) in two breast tumors with (ST23784/17) and without (Ti41749/17) amplification of stemness genes

Journal: Oncotarget

Article Title: Amplifications of stemness genes and the capacity of breast tumors for metastasis

doi: 10.18632/oncotarget.27608

Figure Lengend Snippet: Stem cell populations (CD326+ fraction) in two breast tumors with (ST23784/17) and without (Ti41749/17) amplification of stemness genes

Article Snippet: The EpCam + cell fraction was isolated using the EasySepTM Human EpCam Positive Selection Kit (StemCell Technologies, Catalog #18356).

Techniques: Amplification, Expressing